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ニューバランス レディース crystallin in selenite cataract ma

crystallin in selenite cataract may result from selenite

Decreased chaperone activity of crystallin in selenite cataract may result from seleniteinduced aggregation

H Yan1,2, J J Harding2, Y N Hui3 and M Y Li4

1Department of Ophthalmology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China2Nuffield Laboratory of Ophthalmology, University of Oxford, Oxford, ニューバランス レディース UK3Institute of Ophthalmology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China4Department of Prosthetics, Stomatological エルメス バッグ Hospital, The Fourth Military Medical University, Xi'an, ChinaCorrespondence: JJ Harding, Nuffield Laboratory of Ophthalmology, University of Oxford, Walton Street, Oakley Oxford OX2 6AW, UK.

Top of pageAbstractPurpose To investigate the role of chaperone activity of crystallin in seleniteinduced cataract formation.

Methods Selenite cataract was induced in Sprague rats by five subcutaneous injections of sodium selenite over a 20day period starting at 8 days postpartum. Crystallin was separated from the rat lenses by sizeexclusion chromatography. Bovine Lcrystallin and Lcrystallin were isolated for studies in vitro, and for the chaperone assays. The protective effects of both H and Lcrystallin were measured spectrophotometrically in four different assay プーマ 店舗 procedures including the thermally induced プーマ ランニング aggregation of catalase and Lcrystallin, and the ニューバランス 996 fructation and heatinduced inactivation of catalase. The bovine Lcrystallin was incubated with different concentrations of sodium selenite for 72 h and then its chaperone activity against heatinduced Lcrystallin aggregation was assayed. The aggregation of selenitetreated Lcrystallin was analysed by molecular sieve highperformance liquid chromatography (HPLC).

Results The protection of Hcrystallin was less than that of Lcrystallin in both normal and cataractous lenses. The chaperone activities エアジョーダン 新作 of both H and Lcrystallin in selenite cataract were decreased compared with normal lenses. The protection provided by both Hcrystallin and Lcrystallin against ニューバランス m1400 the thermal aggregation of catalase was much greater than their protection against thermally and chemically induced inactivation. HPLC analysis demonstrated aggregation of crystallin by sodium selenite after 24 h incubation in a バーバリー 財布 dosedependent fashion.

Conclusion The chaperone activity of crystallin presented parallel patterns of activity with different methods, further supporting the view that the different assays ニューバランス 574 measure essentially the same property. The decreased chaperone activity of crystallin in selenite cataract may result from seleniteinduced aggregation.. 相关的主题文章:

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